Subhash Verma, PhD
Research Associate

202B Johnson Pavilion
3610 Hamilton Walk
Philadelphia, PA 19104-6076
(215) 746-0116

vermas@mail.med.upenn.edu

At Pat's place
At Stanford
Deepawali at my place
During Herpesvirus meeting
Group
With Jason & Ke
Holi at My place
Me at DC
Recieving Young Scientist award
Snow at Philly
With Eliot

Understanding the latent replication mechanism of the Kaposi’ sarcoma associated herpesvirus.

2002-2002

2002-2006

2006-Current:

Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan (05/02-07/02) Mentor: Erle S. Robertson, Ph.D.

Department of Microbiology, University of Pennsylvania, School of Medicine and Abramson Comprehensive Cancer Center, Philadelphia, PA (08/02-07/06) Mentor: Erle S. Robertson, Ph.D.

Research Associate, Department of Microbiology, University of Pennsylvania, School of Medicine, Philadelphia, PA (08/06- Current) Mentor: Erle S. Robertson Ph.D.

1992-1995

1995-1997

1998-2002

Dr. RMLA University, Faizabad, India. Obtained Bachelor Degree in Biology

Dr. RMLA University, Faizabad, India. Obtained Master Degree in Microbiology.

School of Biotechnology, Banaras Hindu University, Varanasi, India. Ph. D. in Biotechnology, Advisor: Prof. Anil K. Tripathi.

Research Fellowship at the International Rice Research Institute, Manila, Philippines.

Graduate Aptitude Test in Engineering (GATE) conducted by University Grant Commission, India.

National Eligibility Test conducted by Council of Scientific and Industrial Research and University Grant Commission, India.

Visiting scientist to the University of Florence under Indo-Italian program for the advancement of science and technology.

Young Scientist award from the Indian Science Congress Association (ISCA).

International Award for Research in Leukemia from Lady Tata Memorial Trust ( London) for the academic Year 2006-2007.

Howard Temin Pathway to Independence Award in Cancer Research (K99/R00) .

1-1K99CA126182-01A1 (Subhash C. Verma) 08/01/07-07/31/2012

Understanding the mechanism of KSHV latent DNA replication

The Major goal of this project is to determine the replication mechanism of KSHV episomes during latent infection. Role of LANA in replication and segregation will be dissected using recombinant KSHV genome. This research will help to understand the replication and passage of virus in tumor cells and thereby using specific inhibitors replication and passage of the virus in the tumor cells can be blocked.

2-LTCS/0398 (Subhash C. Verma) 10/01/06-09/30/07

Lady Tata Memorial Trust- International Awards for Research in Leukemia

Publications and Presentations

Publications in Scientific journals:

1. Anjali Tikko, A. K. Tripathi, Subhash C. Verma, N. Agrawal and Gopal Nath (2001). Application of PCR fingerprinting techniques for identification and discrimination of Salmonella isolates. Current Science, 80, 8, 1049-1052.
2. Subhash C. Verma, J. K. Ladha and A. K. Tripathi (2001). Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deepwater rice. Journal of Biotechnology, 91: 127-141.
3. Anil. K. Tripathi, T. Nagarajan, Subhash C. Verma,D. Le Rudulier (2002). Inhibition of biosynthesis and activity of Nitrogenase in Azospirillum brasilense sp7 under salinity stress. Current Microbiology, 44:363-367.
4. Anil K. Tripathi, Subhash C. Verma and Eliroa Ron (2002). Molecular characterization of bacterial community of rice rhizosphere. Research in Microbiology. 153, 9: 579-584.
5. Subhash C. Verma, Erle S. Robertson (2003). Molecular biology and pathogenesis of Kaposi-sarcoma associated herpesvirus. FEMS Microbiology Letters, 222: 155-163.
6. Subhash C. Verma and Erle S. Robertson (2003). ORF73 encoded by Herpesvirus saimiri strain C488 tethers the viral genome to metaphase chromosomes and binds to cis-acting DNA sequences in the terminal repeats. Journal of Virology Dec; 77 (23):12494-506.
7. Subhash C. Verma, Soumitra P. Choudhury and Anil K. Tripathi (2004). Phylogeny based on 16S rDNA and nifH sequences of Ralstonia taiwanensis strains isolated from North India. Canadian J. of Microbiology May; 50 (5):313-22.
8. Ghsoh, M., Verma, S.C., Mengoni, A., and Tripathi, A.K. (2004). Enrichment and identification of bacteria capable of reducing chemical oxygen demand of anaerobically treated spentwash. Journal of Applied Microbiology 96 (6):1278-86.
9. Subhash C. Verma, Anamika Singh, Anil K, Tripathi (2004). Endophytic colonization ability of two deep water rice endophytes Pantoea sp. and Ochrobactrum sp. using GFP reporter gene. Biotechnology Letters; 26 (5):425-9.
10. Ke Lan, Daniel A. Kuppers, Subhash C. Verma and Erle S. Robertson (2004). Kaposi’s sarcoma associated Herpesvirus encoded Latency Associated Nuclear Antigen inhibits Lytic replication by targeting Rta: A potential mechanism for viral mediated control of latency. Journal of Virology Jun; 78(12):6585-94.
11. Subhash C. Verma, Sumit Borah andErle S. Robertson (2004). The Latency Associated Nuclear Antigen of Kaposi’s sarcoma Associated Herpesvirus up-regulates Sp1 Mediated Transcription of human Telomerase Reverse Transcriptase (hTERT) promoter. Journal of VirologyOct; 78(19):10348-59 .
12. Sumit Borah, Subhash C. Verma and Erle S. Robertson (2004). The ORf73 of Herpesvirus saimiri, a viral homolog of Kaposi’s Sarcoma Associated herpesvirus, modulates the two cellular tumor suppressor proteins p53 and pRb. Journal of VirologyOct; 78 (19):10336-47.
13. S. P. Chowdhury, S. Khanna, Subhash C Verma and Anil K Tripathi (2004). Molecular diversity of tannic acid degrading bacteria isolated from tannery soil. J. Appl Microbiology 2004; 97 (6):1210-9.
14. Ke Lan, D. A. Kuppers, Subhash C. Verma, Nikhil Sharma, Masanao Murakami and Erle S. Robertson (2005). Induction of KSHV Latency Associated Nuclear Antigen by the Lytic Transactivator RTA: A Novel Mechanism for Establishment of Latency. Journal of Virology, June 79 (12): 7453-65.
15. Rajeev Kaul, Subhash C. Verma, Masanao Murakami, Ke Lan, Tathagata Choudhuri, Erle S. (2006). Robertson Epstein - Barr virus protein can upregulate Cyclooxygenase-2 expression through Association with the Suppressor of Metastasis Nm23-H). Journal of Virology, Feb 80 (3): 1321-31.
16. Subhash C. Verma, Tathagata Choudhuri, Rajeev Kaul and Erle S. Robertson (2006). Latency-Associated Nuclear Antigen (LANA) of Kaposi's Sarcoma-Associated herpesvirus interacts with origin recognition complexes at the LANA binding sequence within the Terminal Repeats. Journal of Virology, March 80 (5): 2243-56.
17. Subhash C. Verma, Ke Lan, Tathagata Choudhuri and Erle S Robertson (2006). KSHV encoded LANA modulates K1 expression through its cis-acting elements within the terminal repeats. Journal of Virology, April 80 (7): 3345-58.
18. Anil K. Tripathi, Subhash C. Verma, Soumitra Paul Chowdhury, Michael Lebuhn, Andreas Gattinger and Michael Schloter (2006). Ochrobactrum oryzae sp. nov., a novel endophytic bacterial species isolated from deepwater rice in India.International Journal of Systematic and Evolutionary Microbiology Jul (56): 1677-1680.
19. Tathagata C, Subhash C. Verma, Ke Lan and Erle S. Robertson (2006). Expression of Alpha v integrin is modulated by the Epstein - Barr virus Nuclear Antigen3C and the Metastasis Suppressor Nm23-H1 through interaction with the GATA 1 and Sp1 Transcription Factors. Virology Jul 20; 351(1):58-72.
20. Bharat G. Bajaj, Subhash C. Verma, Ke Lan, Murray A. Cotter, Zenda L. Woodman and Erle S. Robertson (2006). KSHV LANA mediated transcriptional upregulation of Pim-1 kinase can contribute to cell survival and proliferation. Virology, Jul 20; 351(1):18-28.
21. Qiliang Cai, Ke Lan, Subhash C. Verma, Huaxin Si, and Erle S. Robertson (2006). The KSHV latent protein LANA interacts with HIF-1 α to upregulate RTA expression in hypoxia: a potential mechanism for latency control in low oxygen. Journal of Virology Aug; 80 (16):7965-75.
22. Huaxin S. Subhash C. Verma and Erle S. Robertson (2006). Proteomic analysis of the Kaposi's sarcoma Associated Herpesvirus Terminal repeats elements binding proteins. Journal of Virology Sept, 80 (18): 9017-9030.
23. Subhash C. Verma, Bharat Bajaj,Qiliang Cai, Todd Seelhammerand Erle S Robertson (2006). Latency Associated Nuclear Antigen of KSHV at the Terminal Repeats and is important for latent persistence of the virus. Journal of Virology, Nov 80 (22): 11178-11190.
24. Qiliang Cai, Jason S Knight, Suhbash C Verma, Philip Zald and Erle S Robertson (2006). Latency-Associated Nuclear Antigen of KSHV recruits EC5S ubiquitin complex mediating degradation of the tumor suppressors VHL and p53. PLOS Pathogens. 2 (10):e116.
25. Subhash C. Verma, and Erle S. Robertson (2007). The Minimal Replicator Element of KSHV Terminal Repeat Supports Replication in a Semi-Conservative Cell Cycle Dependent Manner. Journal of Virology81(7), 3402-3413.
26. Tathagata Choudhuri, Subhash C. Verma, Ke Lan and Erle S. Robertson (2007). The ATM/ATR signaling effector Chk2 is targeted by Epstein-Barr virus nuclear antigen 3C to release the G2/M cell cycle block. Journal of Virology 81(12), 6718-30.
27. Rajeev Kaul, Subhash C. Verma and Erle S. Robertson (2007). Protein complexes associated with the Kaposi's sarcoma-associated herpesvirus-encoded LANA. Virology364(2), 317-29.
28. Subhash C. Verma , Ke Lan, Tathagata Choudhuri Murray A. Cotter and Erle S Robertson (2007). An Autonomous Replicating Element within the KSHV Genome. Cell Host & Microbe2(2), 106-118.
29. Ke Lan, Subhash C. Verma, Masanao Murakami, Bharat Bajaj, Rajeev Kaul and Erle S. Robertson (2007). KSHV Encoded LANA stabilizes Intracellular Activated Notch by Targeting the F-box Protein Sel10. Proc Natl Acad Sci U S A104:16287-92.

Book Chapters:

• Knight JS, Verma SC, Lan K, Robertson ES (2005). Molecular genetics of herpesviruses: a recombinant technology approach. Methods Mol Biol (Humana Press). 2005; 292:333-52
• Subhash C. Verma, Ke Lan, Zenda L Woodman and Erle S. Robertson (2005). Developments in understanding the mechanism of tumorigenesis mediated by Kaposi’s sarcoma Associated Herpesvirus. Progress in Virus Research edited by Sabine Thebault (Institut de Biologie, Montpellier, France), 2005. 1-59454-438-7.
• Subhash C. Verma, Ke Lan and Erle S Robertson (2005). Structure and Function of Latency Associated Nuclear Antigen (LANA). Kaposi Sarcoma edited by Chris Boschoff. Current Topics Microbiol. Immunol. 2006. 312: p. 101-136.
• Ke Lan, Subhash Verma and Erle S. Robertson (2006). Epstein - Barr virus: Infection, Propagation, Quantitation and Storage. Current Protocols in Microbiology (In Press).
• Subhash C. Verma, Qiliang Cai, Bharat Bajaj and Erle S Robertson (2007). Overview of large DNA tumor virus. DNA Tumor Virus Eds. Blossom Damania and Jim Pipas.

Presentation/participation in National /International Meetings/conferences:

• 3 rd Working Group Meeting on assessing opportunities for Nitrogen Fixation in Rice; IRRI, Manila, Philippines, Aug 9-12, 1999.
• Workshop on molecular tools for the identification and phylogenetic analysis of Bacteria, Banaras Hindu University, Varanasi, India.
• Subhash C. Verma, 88 th session of Indian Science Congress, held at Indian Agricultural Research Institute (IARI), New Delhi, January 3-7, 2001.
• Subhash C. Verma, Molecular analysis of endophytic plant growth promoting bacteria in deep water rice in ECOLOGIA MICROBICA MOLECULARE held at University of Florence, Florence, Italy, Oral Presentation, June 28, 2001.
• Subhash C. Verma. Molecular diversity of endophytic diazotrophs in deep-water rice” in 89 th session of Indian Science Congress Association held at Lucknow University, Lucknow, Oral presentation, Jan 3-7 2002.
• Subhash C. Verma and Erle S. Robertson. Role of Latency Associated Nuclear Antigen (LANA) in Kaposi-sarcoma Associated Herpesvirus (KSHV) mediated oncogenesis. The Eunice and Irving Leopold Annual Scientific Symposium and Retreat. Abramson Cancer Center of the University of Pennsylvania. Poster session. April 2003.
• Subhash C. Verma and Erle S. Robertson. The Open Reading Frame 73 Encoded Protein of Herpesvirus saimiri has Functional Similarity to KSHV Latency Associated Nuclear Antigen Protein. 28 th International Herpesvirus Workshop, Madison, Wisconsin, Poster Session, July 2003.
• Subhash C. Verma and Erle S. Robertson. KSHV encoded Latency Associated Nuclear Antigen (LANA): A multi Functional Protein. University of Pennsylvania, Virology Seminar, Oral Presentation. October 2003.
• Subhash C. Verma and Erle S. Robertson. The Latency Associated Nuclear Antigen of Kaposi’s sarcoma Associated Herpesvirus up-regulates Sp1 Mediated Transcription of human Telomerase Reverse Transcriptase (hTERT) promoter. The Eunice and Irving Leopold Annual Scientific Symposium and Retreat. Abramson Cancer Center of the University of Pennsylvania. Poster session. March 2004.
• Subhash C. Verma and Erle S. Robertson, Maintenance and Replication of Kaposi’s Sarcoma Associated Herpesvirus. 4 th Annual Herpesvirus Symposia, University of Pennsylvania, Oral presentation. June 2004.
• Subhash C. Verma and Erle S. Robertson, Modulation of Telomerase hTERT by LANA through interaction with Sp1. Oral presentation at7 th International Workshop on KSHV held at University of California Santa Cruz (UCSC), oral presentation, Aug 21-25, 2004.
• Subhash C. Verma and Erle S. Robertson. Maintenance and Replication of Kaposi’s Sarcoma Associated Herpesvirus. University of Pennsylvania, Virology Seminar, Oral Presentation. October 12, 2004.
• Subhash C. Verma and Erle S. Robertson. Latency-associated nuclear antigen (LANA) of Kaposi's sarcoma-associated herpes virus interacts with origin recognition complexes at the LANA binding sequence within the terminal repeats. Oral presentation at Tumor Virology Program of Abramson Cancer Center of University of Pennsylvania, Jan 28, 2005.
• Subhash C. Verma and Erle S Robertson. Autonomous Replicating element in KSHV genome. 5 th Annual Herpesvirus Symposia, University of Pennsylvania. Oral Presentation June 2005.
• Subhash C. Verma and Erle S. Robertson. Kaposi's sarcoma-associated herpesvirus-encoded latency-associated nuclear antigen modulates K1 expression through its cis-acting elements within the terminal repeats. Oral presentation at Tumor Virology program of Abramson Cancer Center, University of Pennsylvania, Dec. 16, 2005.
• Subhash C. Verma and Erle S. Robertson. Autonomously Replicating element in KSHV genome: - an alternative mechanism of Herpesviral DNA replication. The Eunice and Irving Leopold Annual Scientific Symposium and Retreat. Abramson Cancer Center of the University of Pennsylvania. Poster session. March 2006.
• Subhash C. Verma, Tathagata Choudhuri, Rajeev Kaul, Nattha Wannissorn and Erle S. Robertson. Latency Associated Nuclear Antigen of Kaposi's sarcoma Associated Herpesvirus Interacts with Origin Recognition Complexes at the LANA Binding Sequence within the Terminal Repeats. Poster presentation at9 th International Workshop on KSHV held at Hyannis, Cape Cod, MA, July 12-15, 2006.
• Subhash C. Verma (2007). An Autonomous Replicating Element in the Kaposi’s sarcoma associated herpesvirus Genome . Henle Symposia of the Tumor Virology Program of the Abramson Cancer Center and the Department of Microbiology, University of Pennsylvania, June 27, 2007.
• Subhash C. Verma and Erle Robertson (2007).Latency Associated Nuclear Antigen of Kaposi’s sarcoma Associated Herpesvirus recruits UNG2 at the terminal repeats and is important for latent persistence of the virus. 10th International Workshop on Kaposi’s sarcoma Associated Herpesvirus (KSHV) and Related Agents. Portland, OR. Aug 1-5, 2007.